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Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles
Published on: August 28, 2015
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Development and characterization of vancomycin-loaded levan-based microparticular system for drug delivery
Ali Demir Sezer1, Hande Kazak Sarılmışer2, Erkan Rayaman3
1a Department of Pharmaceutical Biotechnology , Faculty of Pharmacy, Marmara University , Haydarpaşa, Istanbul , Turkey.
Pharmaceutical Development and Technology
|November 27, 2015
Summary
Biodegradable levan microparticles effectively encapsulate vancomycin (VANCO), showing controlled release and bactericidal activity. This levan-based system demonstrates potential as a safe antibiotic drug carrier.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Microbiology
Background:
- Vancomycin (VANCO) is a critical antibiotic for treating serious bacterial infections.
- Developing effective and safe drug delivery systems is essential for improving therapeutic outcomes.
- Biodegradable polymers offer promising platforms for controlled drug release.
Purpose of the Study:
- To encapsulate vancomycin (VANCO) into biodegradable levan microparticles.
- To optimize the encapsulation process and characterize the resulting microparticles.
- To evaluate the in vitro release kinetics, antibacterial activity, and cytotoxicity of VANCO-loaded levan microparticles.
Main Methods:
- Levan polysaccharide produced by Halomonas smyrnensis AAD6T was used to prepare microparticles via a precipitation method.
- Encapsulation efficiency was optimized by varying drug and polymer concentrations and preparation rotation speed.
- Particle size, surface charge, drug encapsulation capacity, in vitro drug release, antibacterial activity against Bacillus subtilis, and cytotoxicity to L929 cells were assessed.
Main Results:
- Levan microparticles ranged from 0.404 to 1.276 μm in size with a surface charge between +4.1 and +6.5 mV.
- The highest drug encapsulation capacity achieved was 74.7%, influenced by polymer concentration.
- In vitro release showed an initial burst effect (10-20%) followed by sustained release fitting the Higuchi model. Released VANCO exhibited bactericidal activity, and the microparticles were non-toxic to L929 cells at tested concentrations.
Conclusions:
- Levan microparticulate systems can be effectively prepared using a simple technique.
- These systems demonstrate controlled release of vancomycin with sustained antibacterial activity.
- Levan microparticles represent a potential and safe carrier for antibiotic drugs like vancomycin.
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