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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

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Related Experiment Video

Updated: Mar 29, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Platform for high-throughput antibody selection using synthetically-designed antibody libraries.

Melissa Batonick¹, Erika G Holland¹, Valeria Busygina¹

¹AxioMx, Inc., 688 East Main Street, Branford, CT 06405, United States.

New Biotechnology

|November 27, 2015

Summary

This summary is machine-generated.

Researchers created a new antibody library using defined regions to better mimic natural human antibodies. This method allows for the selection of specific antibodies against disease targets.

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Last Updated: Mar 29, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

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Published on: January 17, 2015

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Area of Science:

Immunology
Biotechnology
Molecular Biology

Background:

Synthetic antibody libraries are crucial for discovering new therapeutics.
Current methods often result in libraries with diversity exceeding natural antibody repertoires.
Achieving practical diversity within the limits of bacterial transformation is a key challenge.

Purpose of the Study:

To develop a synthetic antibody library with diversity constrained to natural human antibody sequences.
To select novel antibodies against specific human protein targets.
To establish a method for reconstructing consensus antibodies from enriched sequences.

Main Methods:

Generation of a single-chain variable fragment (scFv) phage library using pre-defined complementarity determining regions (CDRs).
Selection of antibodies against four distinct human protein targets.
Analysis of enriched sequences at CDRs in early selection rounds.

Main Results:

A scFv phage library with constrained diversity was successfully generated.
Novel antibodies with selectivity for human protein targets were identified.
Reconstruction of a consensus antibody from early-round CDR sequences was demonstrated.

Conclusions:

Constraining synthetic library diversity to pre-defined CDRs can more closely mimic natural antibody repertoires.
This approach enables efficient selection of specific antibodies.
Early identification of enriched CDR sequences facilitates consensus antibody reconstruction.