Related Experiment Video
Updated: Mar 29, 2026

Establishment of Hepatocarcinoma in BALB/c-nu Mice and Investigation of the Therapeutic Effect of the Sanleng Jiashen Formula
Published on: January 26, 2024
South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anticancer Splicing Activity
Zodwa Dlamini1, Zukile Mbita2, David O Bates3
1Research, Innovation & Engagements, Mangosuthu University of Technology, 392, Durban, 4031, South Africa. dlaminiz@mut.ac.za.
Abstract:
RT-PCR is an invaluable tool for the detection and characterization of mRNA. Cancer cell lines are treated with crude plant extracts and RNA is extracted and purified with DNase prior to RT-PCR. RT-PCR first-strand cDNA synthesis is done using random primers and can be refrigerated at 4 °C. PCR from the stored cDNA is performed using transcript-specific primers and electrophoresed on a molecular grade agarose gel to separate the splice variants.

