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Updated: Mar 29, 2026
Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
Published on: February 25, 2021
Effect of charge ratio on lipoplex-mediated gene delivery and liver toxicity
Jamie L Betker1, Thomas J Anchordoquy1
1University of Colorado, Skaggs School of Pharmacy & Pharmaceutical Sciences, 12850 E. Montview Blvd., Aurora, CO 80045, USA.
Background:
The vast majority of studies investigating gene delivery have utilized cationic delivery vehicles, but anionic nanoparticles can also possess high transfection activity, and offer significant benefits in terms of ease of preparation and reduced toxicity.
Results:
Our study on lipoplexes possessing cholesterol nanodomains demonstrates that in vitro transfection after exposure to serum can be high at anionic charge ratios, and that this effect is also evident in studies assessing delivery to tumors in vivo, despite reduced circulation times. In addition, accumulation in the liver and lungs is reduced as compared with lipoplexes formulated at cationic charge ratios.
Conclusion:
Lipoplexes prepared at anionic charge ratios offer comparable tumor delivery and reduced liver toxicity despite shorter circulation times.
