Reversed-phase ion-pair high-performance liquid chromatography assay of polyprenyl diphosphate oligomer homologues
Vyacheslav V Kozlov1, Leonid L Danilov2
1Laboratory of natural immunity, Department of immunology, N.F. Gamaleya Federal Research Centre of Epidemiology and Microbiology, Moscow, Russian Federation.
Abstract:
A reversed-phase ion-pair high-performance liquid chromatography procedure was developed for the separation of polyprenyl diphosphate oligomer homologues obtained chemically from plant polyprenols. Tetrabutylammonium phosphate was used as the ion-pair reagent, and the dependence of the separation quality on pH of ion-pair reagent was investigated for the first time. The procedure is applicable for the control of commercial available polyprenyl monophosphates (the active components of veterinary drugs Phosprenyl and Gamapren) for the possible presence of polyprenyl diphosphate byproducts.
