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Euthanasia using gaseous agents in laboratory rodents.

A M Valentim1, S R Guedes2, A M Pereira2

  • 1Laboratory Animal Science, Institute of Molecular and Cell Biology (IBMC), University of Porto, Rua do Campo Alegre, Porto, Portugal Institute for Research and Innovation in Health (i3S), University of Porto, Rua Alfredo Allen, Porto, Portugal.

Laboratory Animals
|November 27, 2015
PubMed
Summary

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This summary is machine-generated.

Carbon dioxide (CO2) euthanasia in rodents is debated for animal welfare. While CO2 is common, alternative gases may be more humane, but more research is needed for definitive conclusions.

Area of Science:

  • Laboratory animal science
  • Veterinary medicine
  • Animal welfare science

Background:

  • Euthanasia methods for laboratory rodents are under scrutiny.
  • Inhaled agents are widely used for mass euthanasia but raise animal welfare concerns.
  • Existing data on gaseous euthanasia agents requires synthesis.

Purpose of the Study:

  • To review and discuss available data on gaseous euthanasia agents for laboratory rodents.
  • To address euthanasia of rodent fetuses and neonates.
  • To highlight inconsistencies and knowledge gaps in current euthanasia literature.

Main Methods:

  • Comprehensive review of scientific literature on inhaled euthanasia agents.
  • Discussion of carbon dioxide (CO2), volatile anesthetics (isoflurane, sevoflurane, halothane, enflurane), carbon monoxide, and inert gases.
Keywords:
carbon dioxideeuthanasialaboratory rodentsvolatile agentswelfare

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  • Inclusion of euthanasia techniques for fetuses and neonates.

    • Main Results:

    • A table summarizing gaseous euthanasia techniques for laboratory rodents was compiled.
    • Insufficient evidence currently exists to ban CO2, but alternative gases show potential for improved humane outcomes.
    • The benefit of pre-anesthetic gas exposure to minimize distress remains unclear.

    Conclusions:

    • Further research is needed to clarify the advantages of specific anesthetic gases over CO2 for rodent euthanasia.
    • Development of methods to minimize distress, such as in-home cage euthanasia, is encouraged.
    • Scientific outcomes must guide euthanasia method selection to uphold the 3Rs (Replacement, Reduction, Refinement).