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Updated: Mar 29, 2026

Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
Published on: August 6, 2012
A highly sensitive strategy for base excision repair enzyme activity detection based on graphene oxide mediated
Qiang Xi1, Jun-Jie Li, Wen-Fang Du
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. rqyu@hnu.edu.cn jianhuijiang@hnu.edu.cn.
Abstract:
A novel fluorescent nanosensor has been developed by combining super fluorescence quenching ability of graphene oxide and hybridization chain reaction amplification, which enables highly sensitive detection of base excision repair enzyme activity with a wide dynamic range from 0.0001 to 100 U mL(-1) and a detection limit of 0.00006 U mL(-1).
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