Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robotic Centrifugal Microfluidics with In-Rotation Liquid Supply for the Extraction of Multiple Liquid Biopsy Analytes in One Platform.

Biosensors·2026
Same author

Centrifugal microfluidic automation of the protein aggregation capture workflow for robust mass spectrometry-based proteomics.

Lab on a chip·2026
Same author

Frailty identifies early and non-cardiac healthcare utilization after cardiovascular hospitalization.

Frontiers in cardiovascular medicine·2026
Same author

Factors associated with frailty in older adults hospitalized for cardiovascular disease: A multidimensional cross-sectional observational study.

Geriatric nursing (New York, N.Y.)·2026
Same author

Integrating molecular tools into leishmaniosis surveillance: evaluation of a commercial qPCR kit in sand fly vectors.

Parasites & vectors·2026
Same author

Nurse-Led Health Education Intervention on Basic Life Support for Relatives of Patients With Cardiac Conditions: A Quasi-Experimental Pilot Study.

Nursing & health sciences·2026

Related Experiment Video

Updated: Mar 29, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.2K

Digital droplet PCR on disk.

Friedrich Schuler1, Martin Trotter2, Marcel Geltman2

  • 1Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany and Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany. Friedrich.Schuler@imtek.uni-freiburg.de.

Lab on a Chip
|November 27, 2015
PubMed
Summary
This summary is machine-generated.

We developed an integrated digital droplet PCR (ddPCR) system on a disposable disk for mass fabrication. This system enables automated sample-to-digital answer analysis for clinical diagnostics.

More Related Videos

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
07:43

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

Published on: July 14, 2023

3.0K
Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.7K

Related Experiment Videos

Last Updated: Mar 29, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

14.2K
Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices
07:43

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

Published on: July 14, 2023

3.0K
Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

5.7K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Microfluidics

Background:

  • Existing digital droplet PCR (ddPCR) systems face challenges with integration and mass fabrication.
  • There is a need for streamlined, cost-effective ddPCR solutions for widespread clinical use.

Purpose of the Study:

  • To present an integrated, mass-fabrication-compatible ddPCR system on a disposable disk.
  • To demonstrate a complete workflow from sample to digital result within a single cartridge.

Main Methods:

  • Centrifugal step emulsification for droplet generation in fluorinated oil.
  • Capillary action for emulsion alignment and bubble removal during PCR and fluorescence readout.
  • Integration of emulsification, PCR, and readout in a single-chamber disposable disk.

Main Results:

  • Successful quantitative detection of the cystic fibrosis mutation p.Phe508del across four orders of magnitude.
  • Demonstrated functionality of the integrated ddPCR process chain for genetic analysis.
  • Ensured stable emulsion positioning and bubble-free readout during thermocycling.

Conclusions:

  • The developed integrated ddPCR disk system is compatible with mass fabrication and simplifies manual handling.
  • This technology has the potential to enable routine clinical diagnosis through highly integrated sample-to-digital-answer PCR systems.
  • The system shows promise for applications like non-invasive prenatal testing (NIPT).