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Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Split protein biosensor assays in molecular pharmacological studies.

Michael C Wehr¹, Moritz J Rossner²

¹Department of Psychiatry, Ludwig Maximilian University of Munich, Nussbaumstr. 7, D-80336 Munich, Germany.

Drug Discovery Today

|November 28, 2015

Summary

Monitoring protein-protein interactions (PPIs) with genetically encoded biosensors is key to understanding cellular signalling in health and disease. These advanced biosensors enable precise PPI monitoring in living cells, opening new drug discovery avenues.

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Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Cellular signalling relies on dynamic protein-protein interactions (PPIs).
Deregulation of PPIs can alter cellular signalling, impacting health and disease.
Monitoring PPIs is crucial for understanding their roles.

Purpose of the Study:

To highlight the utility of genetically encoded biosensors for monitoring PPIs in living cells.
To discuss the application of split protein biosensors for precise and robust PPI analysis.
To explore how advanced biosensor platforms can advance drug discovery.

Main Methods:

Utilizing genetically encoded biosensors based on protein fragment complementation.
Employing split protein biosensors with fluorescent proteins or luciferases.
Leveraging split TEV and split ubiquitin biosensor platforms with transcriptional barcode reporters.

Main Results:

Genetically encoded biosensors enable precise and robust monitoring of PPIs in living cells.
Split protein biosensors, including fluorescent and luciferase-based systems, are widely applied.
Advanced platforms offer flexible readouts amenable to high-throughput formats and next-generation sequencing.

Conclusions:

Combining biosensor technologies allows for parallel assessment of drug target activities and cellular response profiles.
These integrated approaches offer new avenues for drug discovery.
Monitoring dynamic PPIs with advanced biosensors is a powerful strategy in biomedical research.