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Related Experiment Video

Updated: Mar 29, 2026

Lentivirus Production
11:42

Lentivirus Production

Published on: October 2, 2009

67.9K

Lentivirus Production and Purification.

Matthew J Benskey1, Fredric P Manfredsson2

  • 1Department of Translational Science and Molecular Medicine, Michigan State University, 333 Bostwick Avenue NE, Grand Rapids, MI, 49503-2532, USA. Matthew.Benskey@hc.msu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2015
PubMed
Summary

This study details a simple protocol for producing lentiviral (LV) vectors, which can integrate genes into dividing and nondividing cells. The method involves triple transfection and purification, yielding high-titer LV vectors for versatile in vitro and in vivo applications.

Keywords:
Gene deliveryGene integrationLentivirusVSV-GViral vector

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Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Biotechnology

Background:

  • Lentiviral (LV) vectors are advanced gene delivery systems.
  • LV vectors enable transgene integration into the host cell genome, including non-dividing cells.
  • This capability offers significant advantages over other gene delivery methods.

Purpose of the Study:

  • To present a straightforward protocol for lentiviral vector production.
  • To describe a method for generating high-titer LV vectors.
  • To offer a versatile protocol adaptable for various research needs.

Main Methods:

  • Triple transfection of HEK-293 cells with LV transfer vector and LV helper plasmids.
  • Purification of lentiviral virions from cell culture media.
  • Adaptable protocol for specific researcher requirements.

Main Results:

  • Successful production of lentiviral vectors.
  • Achieved relatively high titers of functional LV vectors.
  • Demonstrated versatility for transducing diverse cell types.

Conclusions:

  • The described protocol provides an accessible method for lentiviral vector production.
  • The protocol yields high-titer LV vectors suitable for broad applications.
  • LV vectors are powerful tools for both in vitro and in vivo gene delivery.