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Related Experiment Videos

Corticosteroid 11 beta-dehydrogenase in rat testis.

D M Phillips1, V Lakshmi, C Monder

  • 1Population Council, New York, New York 10021.

Endocrinology
|July 1, 1989
PubMed
Summary

Corticosteroid 11 beta-dehydrogenase, an enzyme in the testis, appears postnatally, localizing to Leydig cells. This enzyme likely protects the testis from excess cortisol, correlating with testosterone rise.

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Area of Science:

  • Reproductive biology
  • Endocrinology
  • Enzymology

Background:

  • Corticosteroid 11 beta-dehydrogenase (11-HSD) converts active cortisol to inactive cortisone.
  • Excess cortisol can impair testicular function in mammals.
  • Understanding 11-HSD distribution in the testis is crucial for reproductive health.

Purpose of the Study:

  • To investigate the cellular localization of 11-HSD in the developing rat testis.
  • To correlate the enzyme's appearance with testicular development and function.

Main Methods:

  • Immunofluorescence staining using purified anti-rat 11-HSD antiserum.
  • Localization in fetal, young, and adult rat testes.
  • Germ cell depletion experiments.

Main Results:

  • 11-HSD was undetectable in fetal and very young rat testes.
  • Enzyme expression appeared around day 26, increasing significantly by day 31.
  • In adult rats, 11-HSD was abundant in the interstitial region, particularly in Leydig cells.
  • Germ cell depletion did not affect 11-HSD fluorescence.

Conclusions:

  • 11-HSD appears in rat Leydig cells postnatally.
  • The enzyme's expression correlates with Leydig cell proliferation and rising testosterone levels.
  • Leydig cell 11-HSD may protect the testis from detrimental cortisol effects.

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