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Measurement of serum interleukin-2 activity
J D Fleischmann1, D B Wentworth, K M Thomas
1Department of Surgery (Urology), Metropolitan General Hospital, Case Western Reserve University, Cleveland, Ohio 44109.
Abstract:
The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 degrees C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 inhibitory activity native to human serum. As this report will demonstrate, however, 30% to 50% of interleukin-2 activity in serum taken from patients or normal volunteers was destroyed by heating at 56 degrees C. No loss of recombinant interleukin-2 activity occurred during heating in serum-free media. The percentage of interleukin-2 activity lost at 56 degrees C varied from patient to patient and also varied with the time of exposure. Native serum interleukin-2 inhibitory activity can be removed, and interleukin-2 activity can be assessed accurately in serial dilutions of the serum beyond 1:64.
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