Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The regulation, function and disease relevance of cytoplasmic tRNAs.

Nature reviews. Molecular cell biology·2026

Same author

Automated high-throughput selection of DNA aptamers using a common optical next-generation sequencer.

Nucleic acids research·2026

Same author

Large variations in total and allele-specific transcript expression in a disease mutation-independent manner.

Scientific reports·2026

Same author

ADAM-tRNA-seq: an optimized approach for demultiplexing and enhanced hierarchal mapping in direct tRNA sequencing.

Nucleic acids research·2026

Same author

mRNA-based immunotherapy platform targeting endometrial cancer.

Journal of translational medicine·2025

Same author

Translon: a single term for translated regions.

Nature methods·2025

Search research articles

Related Experiment Video

Updated: Mar 29, 2026

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

Quantifying the 'escapers' among RNA species.

Iolanda Ferro¹, Zoya Ignatova²

¹Biochemistry and Molecular Biology, Dept. of Chemistry and Biochemistry, University of Hamburg, Hamburg 20146, Germany.

Biochemical Society Transactions

|November 29, 2015

Summary

Transfer RNAs (tRNAs) are crucial for protein synthesis and cellular function. Their variable abundance impacts cellular processes and proteome, with new deep-sequencing methods offering cell-wide resolution for study.

Keywords:

ribonucleic acid transfer ribonucleic acid (tRNA)transfer ribonucleic acid (tRNA) modification transfer ribonucleic acid (tRNA)-sequencing translation

More Related Videos

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

Related Experiment Videos

Last Updated: Mar 29, 2026

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells

Published on: November 21, 2025

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

Area of Science:

Molecular Biology
Genetics
Cellular Biology

Background:

Transfer RNAs (tRNAs) are essential molecules for protein translation.
Emerging research indicates tRNAs play significant roles beyond translation in various cellular processes.
tRNA abundance is not constant but highly variable and adaptable across species and within different cell types or conditions.

Purpose of the Study:

To review current methodologies for identifying and quantifying tRNAs.
To assess the functional integrity of tRNAs using various approaches.
To highlight novel deep-sequencing technologies for cell-wide tRNA analysis.

Main Methods:

Review of existing techniques for tRNA identification and quantification.
Discussion of methods assessing tRNA functional integrity.
Exploration of deep-sequencing based approaches for high-resolution tRNA analysis.

Main Results:

tRNA gene numbers vary significantly between species.
tRNA abundance is dynamically regulated and influences proteome in a cell- and tissue-specific manner.
Deep-sequencing technologies provide unprecedented cell-wide resolution for studying tRNAs.

Conclusions:

Understanding tRNA abundance and regulation is critical for comprehending cellular function.
A range of methods exist for tRNA analysis, with deep sequencing offering advanced capabilities.
Further research into tRNA quantification and integrity is essential for uncovering their full biological roles.