Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fixing Double-strand Breaks

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...

Fixing Double-strand Breaks

Fixing Double-strand Breaks

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

Nucleotide Excision Repair

Nucleotide Excision Repair

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CIP2A recruits SLX4-MUS81-XPF in mitosis and protects against replication stress.

EMBO reports·2026

Same author

Ribosome biogenesis is increased in hepatocellular carcinoma and represents a potential therapeutic target.

NAR cancer·2026

Same author

ARID1A stabilizes non-homologous end joining factors at DNA breaks induced by the G4 ligand pyridostatin.

Cell reports·2025

Same author

A High-Throughput ImmunoHistoFluorescence (IHF) Method for Sub-Nuclear Protein Analysis in Tissue.

Cells·2025

Same author

Alpha-synuclein regulates nucleolar DNA double-strand break repair in melanoma.

Science advances·2025

Same author

MDC1 mediates Pellino recruitment to sites of DNA double-strand breaks.

Life science alliance·2025

Search research articles

Related Experiment Video

Updated: Mar 29, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

Nucleolar responses to DNA double-strand breaks.

Dorthe Helena Larsen¹, Manuel Stucki²

¹Danish Cancer Society Research Center, Strandboulevarden 49, 2100 Copenhagen, Denmark dhl@cancer.dk.

Nucleic Acids Research

|November 29, 2015

Summary

The DNA damage response (DDR), orchestrated by ATM/ATR kinases, maintains genome stability. Recent findings reveal the DDR

More Related Videos

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Related Experiment Videos

Last Updated: Mar 29, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Area of Science:

Cellular Biology
Molecular Biology
Genetics

Background:

Cellular homeostasis is crucial for preventing disease and transformation.
The DNA damage response (DDR), involving ATM/ATR kinases, is vital for genome stability.
The DDR influences transcription, splicing, and metabolism.

Purpose of the Study:

To review recent findings on the DDR's role within nucleoli.
To explore how the DDR regulates transcription and DNA double-strand break (DSB) repair in the nucleolus.

Main Methods:

Review of recent scientific literature.
Analysis of studies investigating DDR signaling pathways.
Examination of research on nucleolar functions in DNA repair and transcription.

Main Results:

The DDR controls nucleolar transcription, even when DNA breaks are not in rDNA repeats.
The DDR is activated by a general nuclear DDR.
Initial insights into the organization of DSB repair within the nucleolus have been gained.

Conclusions:

Recent studies provide a comprehensive view of the DDR's role in maintaining cellular homeostasis.
The DDR significantly regulates fundamental cellular processes, including transcription and repair, within the nucleolus.