Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

15.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
15.2K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

4.8K
4.8K
Types of RNA01:23

Types of RNA

73.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
73.8K
Types of RNA01:20

Types of RNA

16.3K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
16.3K
Types of RNA01:20

Types of RNA

2.1K
2.1K
Types of RNA01:23

Types of RNA

30.5K
30.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacillus subtilis 6S-1 RNA regulates transcription of genes related to surfactin biosynthesis.

Biochimie·2025
Same author

Erratum to: Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines.

Doklady. Biochemistry and biophysics·2023
Same author

Expression of CASC8 RNA in Human Pancreatic Cancer Cell Lines.

Doklady. Biochemistry and biophysics·2022
Same author

Modified Oligonucleotides: New Structures, New Properties, and New Spheres of Application.

Russian journal of bioorganic chemistry·2021
Same author

[MutL Protein from the Neisseria gonorrhoeae Mismatch Repair System: Interaction with ATP and DNA].

Molekuliarnaia biologiia·2021
Same author

Comparative Analysis of Long Noncoding RNA Expression in Human Hepatocyte Cell Lines and Liver.

Doklady. Biochemistry and biophysics·2020

Related Experiment Video

Updated: Mar 29, 2026

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.2K

Small Noncoding 6S RNAs of Bacteria.

O Y Burenina1, D A Elkina, R K Hartmann

  • 1Lomonosov Moscow State University, Chemistry Department, Moscow, 119991, Russia.

Biochemistry. Biokhimiia
|November 30, 2015
PubMed
Summary
This summary is machine-generated.

Small noncoding RNAs (ncRNAs), like bacterial 6S RNA, regulate gene transcription by binding RNA polymerase. Product RNA synthesis releases the polymerase, enabling reversible gene expression control.

More Related Videos

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.6K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.8K

Related Experiment Videos

Last Updated: Mar 29, 2026

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
06:30

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Published on: January 27, 2021

2.2K
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

7.6K
Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

24.8K

Area of Science:

  • Bacterial molecular biology
  • Gene regulation
  • Noncoding RNA function

Background:

  • Small noncoding RNAs (ncRNAs) regulate diverse cellular processes in eukaryotes and prokaryotes.
  • 6S RNA, found in all bacteria, is a well-characterized prokaryotic ncRNA.
  • 6S RNA inhibits bacterial RNA polymerase (RNAP) holoenzymes, impacting hundreds of genes.

Purpose of the Study:

  • To review the main features and functions of bacterial 6S RNAs.
  • To focus on the peculiarities of product RNA (pRNA) synthesis.
  • To highlight the evolutionary significance of ncRNA-mediated RNAP regulation.

Main Methods:

  • Comparative transcriptome analysis to identify 6S RNA-regulated genes.
  • Biochemical studies on 6S RNA-RNAP interactions.
  • Analysis of pRNA synthesis and its role in releasing RNAP.

Main Results:

  • 6S RNA possesses a conserved secondary structure that binds and inhibits RNAP holoenzymes.
  • 6S RNA serves as a template for pRNA synthesis, which is crucial for releasing RNAP and reversing transcriptional inhibition.
  • Regulation by 6S RNA affects hundreds of genes involved in various cellular processes.
  • A similar mechanism exists for mouse B2 RNA, suggesting a common evolutionary origin.

Conclusions:

  • 6S RNA is a key regulator of bacterial transcription through RNAP inhibition and pRNA-mediated release.
  • The synthesis of pRNAs is vital for the dynamic control of gene expression.
  • ncRNA-dependent regulation of RNAP is evolutionarily conserved and fundamentally important for cellular function.