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Occurrence and characterization of inducible clindamycin resistance in canine methicillin-resistant Staphylococcus

Pattrarat Chanchaithong1, Nuvee Prapasarakul1

  • 1Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan District, Bangkok 10330, Thailand.

Veterinary Journal (London, England : 1997)
|December 1, 2015
PubMed

Insights

Inducible clindamycin resistance was found in 5% of Staphylococcus pseudintermedius from dogs in Thailand. D-zone testing is recommended for methicillin-resistant Staphylococcus pseudintermedius (MRSP) to ensure correct antibiotic choices.

Area of Science:

  • Veterinary Microbiology
  • Antimicrobial Resistance
  • Molecular Epidemiology

Background:

  • Staphylococcus pseudintermedius is a common canine pathogen.
  • Methicillin-resistant Staphylococcus pseudintermedius (MRSP) poses a significant veterinary challenge.
  • Accurate susceptibility testing for clindamycin is crucial for effective treatment.

Purpose of the Study:

  • To detect inducible clindamycin (iCLI) resistance in Staphylococcus pseudintermedius from dogs in Thailand.
  • To characterize iCLI-resistant strains using molecular methods.
  • To evaluate the utility of D-zone testing for identifying iCLI resistance.

Main Methods:

  • D-zone testing was performed on Staphylococcus pseudintermedius isolates.
  • Molecular typing included pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).
  • Antibiograms were determined for characterized isolates.

Main Results:

  • Inducible clindamycin resistance was detected in 10/200 (5%) S. pseudintermedius isolates from 7/41 (17%) dogs.
  • All resistant isolates were methicillin-resistant Staphylococcus pseudintermedius (MRSP) and multidrug-resistant.
  • Resistant MRSP strains harbored the erm(B) gene, showed similar PFGE patterns, and belonged to clonal complex 112.

Conclusions:

  • D-zone testing is effective in detecting inducible clindamycin resistance in canine S. pseudintermedius.
  • The study identified a concerning prevalence of iCLI-resistant MRSP in Thailand.
  • Implementing D-zone testing is recommended to prevent misinterpretation of clindamycin susceptibility and control the spread of resistant bacteria.
Keywords:
D-zone testDogInducible clindamycin resistanceMethicillin-resistant Staphylococcus pseudintermediusMultidrug resistance

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