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Updated: Mar 29, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Liposome-Loaded Cell Backpacks.

Roberta Polak^1,2, Rosanna M Lim³, Marisa M Beppu⁴

¹School of Pharmaceutical Sciences, University of Sao Paulo, USP Sao Paulo, SP, 05508-900, Brazil.

Advanced Healthcare Materials

|December 1, 2015

Summary

Cell backpacks, micron-scale patches for targeted drug delivery, were enhanced with echogenic liposomes (ELIPs) carrying doxorubicin (DOX). This method significantly increased drug loading and showed potential for cellular drug delivery applications.

Keywords:

cell backpacks doxorubicin echogenic liposomes multilayer films targeted drug delivery

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Published on: August 31, 2019

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On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Area of Science:

Biomaterials Science
Nanotechnology
Drug Delivery

Background:

Cell backpacks are micron-scale patches fabricated by layer-by-layer (LbL) assembly.
They are explored as potential vehicles for cellular-level targeted drug delivery.
Echogenic liposomes (ELIPs) are utilized for drug encapsulation.

Purpose of the Study:

To embed echogenic liposomes (ELIPs) containing doxorubicin (DOX) into cell backpacks using LbL assembly.
To evaluate the efficiency of ELIP incorporation and its impact on drug loading capacity.
To assess the viability of monocyte-backpack conjugates as drug delivery systems.

Main Methods:

Fabrication of cell backpacks using layer-by-layer (LbL) assembly.
Incorporation of doxorubicin (DOX)-loaded echogenic liposomes (ELIPs) via electrostatic interactions.
Evaluation of film systems, including Poly(allylamine hydrochloride)/poly(acrylic acid) (PAH/PAA)n and Poly(diallyldimethylammonium chloride)/poly(styrene sulfonate) (PDAC/SPS)n, for ELIP incorporation and structural integrity.
Cytotoxicity studies on monocyte backpack conjugates.

Main Results:

Polymer film systems like (PAH/PAA)n and (PDAC/SPS)n demonstrated high ELIP incorporation while preserving vesicle integrity.
ELIP-containing backpacks achieved up to three times greater doxorubicin (DOX) loading compared to those without ELIPs.
Monocyte backpack conjugates remained viable for up to 72 hours, indicating their potential as drug delivery vehicles.

Conclusions:

Echogenic liposome (ELIP) incorporation into cell backpacks via LbL assembly significantly enhances drug loading capacity.
The developed ELIP-containing backpacks show promise as versatile and viable drug delivery vehicles for cellular applications.
This approach broadens the potential applications of cell backpacks in targeted therapy.