Application of a high-throughput, parallel HPLC system for quantitative chiral analysis of pantoprazole
Hao Wang1, Yantong Sun2, Yan Yang1
1College of Life Sciences, Jilin University, Changchun 130012, PR China.
Background:
Chromatographic separation of enantiomers is considered a task in analytical chemistry particularly for high sample throughput. This paper describes a high-throughput parallel HPLC-MS/MS method for the determination of pantoprazole enantiomers.
Results:
Baseline separation of pantoprazole enantiomers was achieved on a Chiralcel OZ-RH column in a run time of 4.5 min. Assays for enantiomers were linear with satisfactory intra- and inter-day precision and accuracy. The assay was suitable for high-throughput analysis as shown by its successful application to a chiral PK study in beagle dog.
Conclusion:
A high-throughput parallel HPLC-MS/MS assay for pantoprazole has been developed and validated. This method provides nearly twofold increased sample throughput, and was shown to be suitable for application in PK studies.
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