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Updated: Mar 29, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
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Single Amino Acid Switch between a Flavin-Dependent Dehalogenase and Nitroreductase.

Arnab Mukherjee1, Steven E Rokita1

  • 1Department of Chemistry, Johns Hopkins University , Baltimore, Maryland 21218, United States.

Journal of the American Chemical Society
|December 1, 2015
PubMed
Summary

A single mutation in a flavoprotein can change its function from dehalogenase to nitroreductase. This discovery aids in developing new catalysts by understanding enzyme evolution and function.

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