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Updated: Mar 29, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Published on: February 5, 2014
Combining the genetic algorithm and successive projection algorithm for the selection of feature wavelengths to
Jun-Hu Cheng1, Da-Wen Sun2, Hongbin Pu1
1College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China.
Abstract:
The potential use of feature wavelengths for predicting drip loss in grass carp fish, as affected by being frozen at -20°C for 24 h and thawed at 4°C for 1, 2, 4, and 6 days, was investigated. Hyperspectral images of frozen-thawed fish were obtained and their corresponding spectra were extracted. Least-squares support vector machine and multiple linear regression (MLR) models were established using five key wavelengths, selected by combining a genetic algorithm and successive projections algorithm, and this showed satisfactory performance in drip loss prediction. The MLR model with a determination coefficient of prediction (R(2)P) of 0.9258, and lower root mean square error estimated by a prediction (RMSEP) of 1.12%, was applied to transfer each pixel of the image and generate the distribution maps of exudation changes. The results confirmed that it is feasible to identify the feature wavelengths using variable selection methods and chemometric analysis for developing on-line multispectral imaging.

