Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.2K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.5K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.5K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.6K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.6K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

5.7K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
5.7K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

42.0K
Overview
42.0K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

13.9K
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteasomal-dependent CHK1 degradation leads to DNA damage accumulation in ALS cellular model systems.

Cell death & disease·2026
Same author

DNA double-strand break response at a glance.

Journal of cell science·2026
Same author

DROSHA, DICER, and damage-induced long ncRNA control BMI1-dependent transcriptional repression at DNA double-strand break.

Cell reports·2025
Same author

DNA damage response defects induced by the formation of TDP-43 and mutant FUS cytoplasmic inclusions and their pharmacological rescue.

Cell death and differentiation·2025
Same author

Traffic light at DSB-transit regulation between gene transcription and DNA repair.

FEBS letters·2024
Same author

TGS1 impacts snRNA 3'-end processing, ameliorates survival motor neuron-dependent neurological phenotypes in vivo and prevents neurodegeneration.

Nucleic acids research·2022

Related Experiment Video

Updated: Mar 29, 2026

Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

89.1K

Non-Coding RNA: Sequence-Specific Guide for Chromatin Modification and DNA Damage Signaling.

Sofia Francia1

  • 1IFOM - FIRC Institute of Molecular Oncology Milan, Italy ; Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche Pavia, Italy.

Frontiers in Genetics
|December 1, 2015
PubMed
Summary

Transcription generates DNA damage but also safeguards genome integrity via RNA molecules. Non-coding RNAs (ncRNAs) influence chromatin, DNA damage response (DDR), and repair at their sites of origin.

Keywords:
DNA-damage responseRNA interferencechromatin modulationnon-coding RNAtranscription

More Related Videos

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.9K
Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
09:29

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

Published on: February 2, 2024

4.4K

Related Experiment Videos

Last Updated: Mar 29, 2026

Chromatin Isolation by RNA Purification ChIRP
11:09

Chromatin Isolation by RNA Purification ChIRP

Published on: March 25, 2012

89.1K
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

Published on: January 31, 2018

10.9K
Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays
09:29

Author Spotlight: Decoding DNA Repair by Extrachromosomal NHEJ Assay and HR Assays

Published on: February 2, 2024

4.4K

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Transcription and DNA damage signaling are intertwined processes affecting genome integrity.
  • RNAs can modulate chromatin conformation, transcription, splicing, and DNA damage response (DDR).
  • The interplay between transcription-induced DNA damage and DDR-mediated transcriptional repression is complex.

Purpose of the Study:

  • To explore the dual role of transcription in genome maintenance.
  • To investigate how RNAs, particularly non-coding RNAs (ncRNAs), influence chromatin, transcription, and DDR.
  • To understand the involvement of the RNA interference (RNAi) machinery in these processes.

Main Methods:

  • Review of experimental evidence on ncRNA function in chromatin modulation.
  • Analysis of studies linking RNAi pathway components to DNA damage response.
  • Examination of research on ncRNAs acting at their genomic loci.

Main Results:

  • RNAs directly impact chromatin structure, transcription, and splicing.
  • RNAs can activate DNA damage response (DDR) and DNA repair pathways.
  • The RNA interference (RNAi) machinery, including ncRNAs, plays a crucial role in regulating chromatin and DDR.
  • ncRNAs modulate chromatin, DDR signaling, and DNA repair at their transcription sites.

Conclusions:

  • Transcription presents a paradox by both threatening and protecting genome integrity.
  • ncRNAs are key regulators acting locally to influence chromatin and DDR.
  • The RNAi pathway is integral to the crosstalk between transcription, chromatin, and DNA repair mechanisms.