Related Experiment Video

Updated: Mar 29, 2026

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
11:32

Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke

Published on: January 3, 2025

1.8K

MicroRNA-323 regulates ischemia/reperfusion injury-induced neuronal cell death by targeting BRI3

Liu Yang1, Yin Xiong1, Xian-Feng Hu1

  • 1Department of Geratology, Pu Ai Hospital of Tongji Medical College, Huazhong University of Science and Technology Wuhan 430033, China.

International Journal of Clinical and Experimental Pathology
|December 1, 2015
PubMed
Abstract

Insights

MicroRNA-323 (miR-323) is upregulated in ischemia/reperfusion (I/R) injury, promoting neuronal cell death by targeting BRI3. Inhibiting miR-323 reduces apoptosis and enhances survival in I/R-injured neurons.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Ischemia/reperfusion (I/R) injury is a significant cause of neuronal damage.
  • MicroRNA-323 (miR-323) has been observed to be upregulated in I/R-affected neuronal cells.
  • The precise role and molecular mechanisms of miR-323 in I/R-induced neuronal cell death require elucidation.

Purpose of the Study:

  • To investigate the function of miR-323 in I/R-induced neuronal cell death.
  • To explore the underlying molecular mechanisms by which miR-323 influences neuronal survival and apoptosis.
  • To determine if miR-323 directly targets specific genes involved in I/R injury.

Main Methods:

  • An in vitro oxygen-glucose deprivation (OGD) model was used to simulate I/R injury in rat hippocampal neurons.
  • Cell apoptosis, cell survival rates, and miR-323 expression levels were measured at various time points post-OGD.
  • miR-323 mimics and anti-miR-323 inhibitors were employed to upregulate or downregulate miR-323 expression, respectively.
  • The interaction between miR-323 and BRI3 was assessed, and the effect of BRI3 knockdown on neuronal cells was evaluated.

Main Results:

  • OGD treatment led to increased apoptosis and decreased survival in rat hippocampal neurons.
  • miR-323 expression levels were significantly elevated following OGD exposure.
  • Upregulation of miR-323 exacerbated apoptosis and reduced cell survival, while miR-323 inhibition had the opposite effect.
  • miR-323 was found to directly bind to the 3'-untranslated region (UTR) of BRI3.
  • Knockdown of BRI3 using siRNA resulted in decreased cell survival and increased apoptosis in neurons.

Conclusions:

  • miR-323 plays a critical role in regulating neuronal cell death following ischemia/reperfusion injury.
  • The pro-apoptotic and anti-survival effects of miR-323 in I/R injury are mediated through its direct targeting of BRI3.
  • Targeting miR-323 or its downstream effector BRI3 may represent a therapeutic strategy for mitigating I/R-induced neuronal damage.
Keywords:
BRI3MicroRNA-323ischemia/reperfusion injuryneuronal apoptosis

More Related Videos

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

1.4K
Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation09:53

Herbal Munziq Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting Inflammation

Published on: January 10, 2025

934

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
9.2K