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Updated: Mar 29, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
MiR-142-3p functions as a potential tumor suppressor directly targeting HMGB1 in non-small-cell lung carcinoma
1Department of Thoracic Surgery, The Third Xiangya Hospital, Central South University No 138 Tong-Zipo Road, Changsha 410013, Hunan Province, P. R. China.
Background:
microRNAs (miRNAs) play a significant role in cancer development and progression by regulating the expression of oncogenes or tumor suppressor genes. Previous study using microarrays demonstrated that miR-142-3p was downregulated in patients with Non-small-cell lung carcinoma (NSCLC). However, the functional role of miR-142-3p in NSCLC is still unclear.
Material And Method:
Real-time quantitative PCR was applied to evaluate the expression level of miRNA-142-3p in NSCLC and normal samples. The cell proliferation of NSCLC cells was analyzed by MTT and colony formation assay after miR-142-3p transfection. Luciferase activities assay, cotransfection and Western blot were used to reveal that the predicted target genes of miR-125b were direct and specific.
Results:
In this study, we demonstrate miR-142-3p was downregulated in NSCLC tissues and cell lines. We demonstrated that the overexpression of miR-142-3p inhibits NSCLC cell proliferation and induced cell apoptosis. Furthermore, we demonstrate HMGB1 was a directly target of miR-142-3p in NSCLC cells, and confirmed the target specificity between miR-142-3p and the HMGB1 3'-untranslated region by luciferase reporter assay.
Conclusions:
These results suggest that miR-142-3p may be a tumor suppressor through the downregulation of HMGB1 in NSCLC. miR-142-3p may be a tumor suppressor and a potential therapeutic agent for patients with NSCLC.
Insights
microRNA-142-3p is downregulated in non-small-cell lung carcinoma (NSCLC). Overexpressing this microRNA inhibits NSCLC cell growth and induces apoptosis, suggesting it acts as a tumor suppressor by targeting HMGB1.
Area of Science:
- Molecular Biology
- Oncology
- Gene Regulation
Background:
- MicroRNAs (miRNAs) are key regulators in cancer, influencing oncogenes and tumor suppressors.
- Previous studies indicated miR-142-3p downregulation in non-small-cell lung carcinoma (NSCLC).
- The precise function of miR-142-3p in NSCLC remained largely undetermined.
Purpose of the Study:
- To investigate the functional role of miR-142-3p in non-small-cell lung carcinoma.
- To identify the molecular targets and mechanisms underlying miR-142-3p's action in NSCLC.
Main Methods:
- Quantitative real-time PCR to assess miR-142-3p expression levels in NSCLC and normal tissues.
- MTT and colony formation assays to evaluate the impact of miR-142-3p on NSCLC cell proliferation.
- Luciferase reporter assays and Western blotting to confirm HMGB1 as a direct target of miR-142-3p.
Main Results:
- miR-142-3p expression was significantly downregulated in NSCLC tissues and cell lines.
- Overexpression of miR-142-3p suppressed NSCLC cell proliferation and promoted apoptosis.
- HMGB1 was identified as a direct target of miR-142-3p in NSCLC, validated by luciferase assays.
Conclusions:
- miR-142-3p functions as a tumor suppressor in NSCLC.
- The tumor-suppressive activity of miR-142-3p is mediated through the downregulation of HMGB1.
- miR-142-3p holds potential as a therapeutic target for non-small-cell lung carcinoma.
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