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Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells

Flora De Conto, Carlo Chezzi, Alessandra Fazzi

    Cellular & Molecular Biology Letters
    |December 1, 2015
    PubMed

    Insights

    Influenza A virus replication is influenced by microtubule dynamics and the ubiquitin-proteasome system. Proteasome activity destabilizes microtubules, favoring virus replication in LLC-MK2 cells but not A549 cells.

    Area of Science:

    • Virology
    • Cell Biology
    • Biochemistry

    Background:

    • Microtubule network dynamics are implicated in the replication of influenza A virus.
    • The role of the ubiquitin-proteasome system in viral replication is not fully understood.

    Purpose of the Study:

    • To investigate the involvement of the ubiquitin-proteasome system in influenza A virus replication.
    • To determine the impact of proteasome activity on microtubule destabilization during infection.

    Main Methods:

    • Infection of LLC-MK2 and A549 cells with influenza A (H1N1) virus.
    • Chemical inhibition and stimulation of proteasome activity.
    • Observation of virus replication rates and microtubule network stability.

    Main Results:

    • In LLC-MK2 cells, proteasome activity destabilized microtubules and enhanced influenza A virus replication.
    • Inhibition of proteasomes partially suppressed virus replication, while stimulation favored it.
    • A549 cells showed minimal impact of proteasome modulators on virus replication.

    Conclusions:

    • Influenza A virus may exploit proteasome functions to create a favorable cytoskeletal environment for replication.
    • The host cell type and virus strain significantly influence the interplay between influenza virus and host proteasome activity.

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