Related Experiment Video
Updated: Mar 29, 2026

Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model
Published on: February 7, 2025
Reconstruction of Long Bone Infections Using the Induced Membrane Technique: Tips and Tricks
Cyril Mauffrey1, Mark E Hake, Vivek Chadayammuri
1*Department of Orthopaedics, Denver Health Medical Center, University of Colorado, School of Medicine, Denver, CO; †Department of Orthopaedic Surgery, University of Michigan Health System, Ann Arbor, MI; and ‡Service de chirurgie orthope[Combining Acute Accent]dique, traumatologique, ho[Combining Circumflex Accent]pital Saint-Antoine, Paris, France.
Unlabelled:
The management of posttraumatic long bone osteomyelitis remains a challenging clinical problem. A systematic approach is necessary, beginning with eradication of the infected bone and soft tissue. There are a number of options for reconstruction of the remaining bone defect, including the induced membrane technique developed by Masquelet. We describe our technique for the 2-stage treatment of long bone osteomyelitis. The first stage involves a radical debridement, stabilization of the bone with either external fixation or an antibiotic-coated intramedullary nail, and placement of a polymethylmethacrylate spacer. The second stage includes excision of the spacer and placement of autologous bone graft. Various resection methods, fixation strategies, antibiotic additives, and types of bone grafts or substitutes can be used. The purpose of our technical article is to share our personal experience and describe several nuances that are critical for the success of this treatment strategy.
Level Of Evidence:
Therapeutic Level IV. See Instructions for Authors for a complete description of levels of evidence.
More Related Videos
09:35Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
09:09Treatment with Vancomycin Loaded Calcium Sulphate and Autogenous Bone in an Improved Rabbit Model of Bone Infection
Published on: March 14, 2019