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Updated: Mar 29, 2026

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Published on: August 4, 2017
Somatic Embryogenesis in Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae)
Miguel P Guerra1, Neusa Steiner2, Francine L Farias-Soares3
1Graduate Program in Plant Genetic Resources, Plant Developmental Physiology and Genetics Laboratory, Federal University of Santa Catarina (UFSC), Florianópolis, 88034-001, SC, Brazil. miguel.guerra@ufsc.br.
Abstract:
This chapter deals with the features of somatic embryogenesis (SE) in Araucaria angustifolia, an endangered and native conifer from south Brazil. In this species SE includes the induction and proliferation of embryogenic cultures composed of pro-embryogenic masses (PEMs), which precede somatic embryos development. A. angustifolia SE model encompasses induction, proliferation, pre-maturation, and maturation steps. Double-staining with acetocarmine and Evan's blue is useful to evaluate the embryonic somatic structures. In this chapter we describe A. angustifolia SE protocols and analyzes morphological features in the different SE developmental stages.
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