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[Interest of Droplet digital PCR in non-invasive prenatal testing]

Lucie Orhant1, Aurélie Vasson1, France Leturcq2

  • 1Service de biochimie et génétique moléculaire, HUPC Hôpital Cochin, Paris, France.

Annales De Biologie Clinique
|December 2, 2015
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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