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Updated: Mar 29, 2026

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Published on: January 1, 2017
Generation of Footprint-Free Induced Pluripotent Stem Cells from Human Fibroblasts Using Episomal Plasmid Vectors
Dmitry A Ovchinnikov1, Jane Sun1, Ernst J Wolvetang2
1Stem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Corner College and Cooper Rds (Building 75), St. Lucia, Brisbane, QLD, 4072, Australia.
Abstract:
Human induced pluripotent stem cells (hiPSCs) have provided novel insights into the etiology of disease and are set to transform regenerative medicine and drug screening over the next decade. The generation of human iPSCs free of a genetic footprint of the reprogramming process is crucial for the realization of these potential uses. Here we describe in detail the generation of human iPSC from control and disease-carrying individuals' fibroblasts using episomal plasmids.
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