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Updated: Mar 29, 2026

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
Published on: September 6, 2014
Isolation of Reprogramming Intermediates During Generation of Induced Pluripotent Stem Cells from Mouse Embryonic
Christian M Nefzger1, Sara Alaei1, Jose M Polo2
1Department of Anatomy and Developmental Biology, Australian Regenerative Medicine Institute, Monash University, Level 3, STRIP 1, Building 75, Wellington Road, Clayton, VIC, 3800, Australia.
Abstract:
Mature cells of the body can be reprogrammed towards a pluripotent state by forced expression of the transcription factors Oct-4, Klf-4, Sox2, and C-Myc (OKSM) at very low efficiency. To study the reprogramming process in detail the rare intermediates of the reaction need to be separated from the bulk population. Using a genetically engineered reprogrammable mouse strain we describe how to isolate intermediates from reprogramming cultures of mouse embryonic fibroblasts via antibody labeling of cell surface markers and fluorescence-activated cell sorting (FACS).
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