Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

17.3K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
17.3K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

1.8K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
1.8K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

86.3K
Overview
86.3K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

8.2K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
8.2K
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

3.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.5K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

18.3K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
18.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conserved T cell receptor usage underpins recognition of CD1c presenting a mycobacterial lipid.

bioRxiv : the preprint server for biology·2026
Same author

A temporal map of B cell diversification mechanisms in mice.

Nature immunology·2026
Same author

CD1a-Mediated Presentation of Canonical Microbial Peptides to T Cells.

bioRxiv : the preprint server for biology·2026
Same author

Specific targeting of MR1-antigen complexes using nanobodies.

bioRxiv : the preprint server for biology·2026
Same author

Protein kinase F regulates the virulence of <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2026
Same author

Sideways lipid presentation by the antigen-presenting molecule CD1c.

Nature communications·2025

Related Experiment Video

Updated: Mar 29, 2026

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
18:08

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation

Published on: December 31, 2007

10.4K

Human autoreactive T cells recognize CD1b and phospholipids.

Ildiko Van Rhijn1, Twan van Berlo2, Tamara Hilmenyuk3

  • 1Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115; Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584CL Utrecht, The Netherlands; bmoody@partners.org mbrenner@partners.org i.vanrhijn@uu.nl.

Proceedings of the National Academy of Sciences of the United States of America
|December 2, 2015
PubMed
Summary

Researchers identified CD1b autoreactive T cells using novel dextramer technology. These T cells recognize phosphatidylglycerol (PG), a lipid antigen found in both self and foreign sources, crucial for detecting infection or cellular stress.

Keywords:
CD1bT celldendritic celllipid antigenself-antigen

More Related Videos

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

6.7K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.3K

Related Experiment Videos

Last Updated: Mar 29, 2026

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
18:08

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation

Published on: December 31, 2007

10.4K
Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
07:48

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

6.7K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.3K

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • CD1b autoreactive T cells are challenging to isolate in humans, unlike T cells recognizing other CD1 proteins or MHC.
  • Understanding CD1b-restricted T cell responses is crucial for immunology and disease research.

Purpose of the Study:

  • To develop a method for identifying CD1b autoreactive T cells in human donors.
  • To identify the specific lipid antigens recognized by CD1b autoreactive T cells.

Main Methods:

  • Development of polyvalent CD1b-protein and carbohydrate backbone (dextramer) complexes.
  • Activation assays using T-cell receptors (TCRs) binding to CD1b-phospholipid complexes.
  • Mass spectrometry and T-cell responses to screen phospholipid classes.

Main Results:

  • Successfully identified CD1b autoreactive T cells from human donors using the developed dextramer technology.
  • Phosphatidylglycerol (PG) was identified as the immunodominant lipid antigen recognized by these T cells.
  • CD1b autoreactive T cells recognized both mammalian and bacterial PG, indicating a dual self and foreign origin recognition.

Conclusions:

  • CD1b autoreactive T cells recognize phosphatidylglycerol (PG), a lipid antigen with both self and foreign origins.
  • This recognition mechanism suggests a broader role in detecting infection- or stress-associated lipids, as PG is abundant in bacteria and mitochondria.
  • The findings challenge traditional models of T-cell recognition, highlighting the importance of CD1b-lipid interactions in immunity.