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Changes in the cytokine expression of peripheral Treg and Th17 cells in children with rotavirus enteritis
Huaifu Dong1, Sehua Qu1, Xin Chen1
1Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, P.R. China.
Insights
Children with rotavirus (RV) enteritis show an imbalance in regulatory T cell (Treg) and T helper 17 (Th17) cells. This immune cell imbalance and altered cytokine levels are significant for understanding RV enteritis pathogenesis.
Area of Science:
- Immunology
- Pediatrics
- Virology
Background:
- Rotavirus (RV) enteritis is a common pediatric illness.
- Immune dysregulation involving T helper 17 (Th17) and regulatory T cells (Treg) may play a role in RV pathogenesis.
Purpose of the Study:
- To investigate changes in peripheral Treg and Th17 cell proportions and their associated cytokines in children with RV enteritis.
- To explore the clinical significance of these immune alterations in RV enteritis.
Main Methods:
- Flow cytometry was used to quantify Treg and Th17 cell proportions in peripheral blood.
- Enzyme-linked immunosorbent assay (ELISA) measured serum levels of cytokines IL-10, TGF-β, IL-17, and IL-6.
Main Results:
- Children with RV enteritis had significantly decreased Treg cell proportions and TGF-β levels compared to controls.
- RV enteritis was associated with significantly increased Th17 cell proportions and elevated IL-17 and IL-6 serum levels.
Conclusions:
- An imbalance in peripheral Treg/Th17 cell populations and cytokine expression occurs in children with RV enteritis.
- Monitoring Treg/Th17 cells and cytokine profiles offers clinical insights into RV enteritis pathogenesis.
Abstract:
The aim of the present study was to investigate the changes in the cytokine expression of peripheral regulatory T cells (Treg) and T helper 17 (Th17) cells in children with rotavirus (RV) enteritis. In total, 102 children with RV enteritis were recruited for the observation group, while 30 healthy cases were included in the control group. Peripheral blood samples were collected from the individuals in the two groups, after which flow cytometry was conducted to detect the proportion of Treg and Th17 cells. In addition, ELISA was used to determine the levels of the cytokines, interleukin (IL)-10, transforming growth factor (TGF)-β, IL-17 and IL-6. When compared with the control group, the proportion of Treg cells and level of TGF-β in the peripheral blood of the children with RV enteritis were significantly decreased (P<0.05); however, the proportion of Th17 cells and the serum levels of IL-17 and IL-6 in the peripheral blood of children with RV enteritis were significantly increased (P<0.05). In conclusion, the present study identified an imbalance in the proportion of peripheral blood Treg/Th17 cells, and subsequently in the expression of cytokines, in children with RV enteritis. Thus, detecting the proportion of peripheral blood Treg/Th17 cells in children with RV enteritis, or the changes in the levels of serum cytokines, is of clinical significance for further investigation into the pathogenesis of RV enteritis.
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