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Published on: July 28, 2012
Effects of siRNA Livin on EJ human bladder cancer cells treated with mitomycin-C
Ya-Hui Song1, Ran Liao1, Peng-Cheng Li1
1Department of Urinary Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China.
Abstract:
The aim of this study was to observe the inhibitory and therapeutic effects of small interfering RNA (siRNA) targeting Livin in EJ human bladder cancer cells. Specific siRNA targeting Livin was synthesized and transfected into EJ human bladder cancer cells treated or not treated with mitomycin-C (MMC). Livin mRNA and protein, as well as proliferation and apoptosis of EJ cells was examined with reverse transcription-polymerase chain reaction, western blotting, Cell Counting Kit-8 assay and flow cytometry, respectively. The results indicated that the expression of Livin mRNA and protein in EJ cells was significantly decreased by siRNA Livin. The proliferation of EJ cells was significantly inhibited by treatment with MMC and transfection of siRNA Livin. The inhibition of cell proliferation by treatment with MMC was further enhanced by transfection of siRNA Livin. The apoptotic rate of cells transfected with siRNA Livin and treated with MMC was significantly higher than those cells receiving a single transfection of siRNA Livin and single treatment of MMC. In conclusion, the present study demonstrates that transfection of siRNA Livin induces growth suppression and apoptosis in EJ human bladder cancer cells, and increases the chemotherapeutic sensitivity of cells to MMC.
Insights
Small interfering RNA (siRNA) targeting Livin suppresses EJ human bladder cancer cell growth and enhances sensitivity to mitomycin-C (MMC). This approach shows promise for improving bladder cancer treatment strategies.
Area of Science:
- Oncology
- Molecular Biology
- RNA Therapeutics
Background:
- Livin is a protein implicated in cancer cell survival and resistance to chemotherapy.
- EJ human bladder cancer cells are a relevant model for studying bladder cancer progression and treatment.
Purpose of the Study:
- To investigate the efficacy of small interfering RNA (siRNA) targeting Livin in EJ human bladder cancer cells.
- To assess the combined effects of siRNA Livin and mitomycin-C (MMC) on cancer cell proliferation and apoptosis.
Main Methods:
- Synthesis and transfection of specific siRNA targeting Livin into EJ cells.
- Quantitative analysis of Livin mRNA and protein expression using RT-PCR and Western blotting.
- Assessment of cell proliferation and apoptosis via Cell Counting Kit-8 assay and flow cytometry.
Main Results:
- siRNA Livin significantly reduced Livin mRNA and protein expression in EJ cells.
- Combined treatment with siRNA Livin and MMC markedly inhibited cell proliferation compared to single treatments.
- The apoptotic rate was significantly elevated in cells treated with both siRNA Livin and MMC.
Conclusions:
- siRNA Livin effectively induces growth suppression and apoptosis in EJ human bladder cancer cells.
- Targeting Livin with siRNA enhances the chemotherapeutic sensitivity of bladder cancer cells to MMC.
- This study highlights the potential of siRNA-based therapies in overcoming chemoresistance in bladder cancer.
