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Updated: Mar 29, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
[HALOTOLERANCE OF ENTEROBACTERIA ISOLATED FROM WATER AND FISH IN THE VOLGA RIVER DELTA]
Abstract:
In the article there are presented the results of a study of halotolerance of enterobacteria isolated from water and fish in the Volga River delta, which were dominant in the microbial landscape of these biotopes. Their halotolerance was studied by means of inoculation of daily pure cultures of meat--peptone broth with 3, 7 and 10% of sodium chloride and incubation at 37°C. At that all studied microflora was found to have significant indices of halotolerance with a predominance of aquatic isolates. At that in 3,0 and 7,0 % NaCl concentrations were very similar, but in 10% NaCl broth the halotolerance of water strain was almost 2.0 times higher than the in fish ones. Among isolated Enterobacteriaceae most halophilic bacteria were from the Proteus group, Citrobacteria and Enterobacteria. The halotolerance of isolated enterobacteria in hydroecosystem of the Volga River delta had seasonal specificity and dynamics. There was established the dynamic growth of this sign in the water and fish strains from spring to autumn. Thus, the analysis of the obtained results of the long-term conducted study has shown the wide dissemination of conditionally pathogenic enterobacteria in water and fish, and their high halotolerance and the ability of some of them to remain viable up to salt production. It gives grounds to recommend the necessity of the introduction of them into the normative documentation in the implementation ofthe sanitary-microbiological control of not only raw materials but also ready salted fish production.
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