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Updated: Mar 29, 2026

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

Recent advances in 2D-LC for bioanalysis.

Dwight R Stoll¹

¹Gustavus Adolphus College, 800 West College Avenue, St. Peter, MN 56082, USA.

|December 3, 2015

Summary

This summary is machine-generated.

Two-dimensional liquid chromatography (2D-LC) has seen major advancements for bioanalysis over the past five years. Its application in characterizing complex protein therapeutics and formulations effectively addresses unique challenges like aggregation.

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

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Published on: May 20, 2013

Area of Science:

Analytical Chemistry
Biochemistry
Pharmaceutical Science

Background:

Two-dimensional liquid chromatography (2D-LC) is a powerful separation technique.
Bioanalysis often involves complex samples, particularly proteins and their formulations.
Characterizing biotherapeutics requires advanced analytical methods due to inherent complexities.

Purpose of the Study:

To summarize key developments in 2D-LC for bioanalysis over the last five years.
To highlight the application of 2D-LC in characterizing protein biotherapeutics and formulations.
To discuss how 2D-LC addresses challenges unique to large biomolecules.

Main Methods:

Review of recent literature on 2D-LC applications in bioanalysis.
Focus on theoretical, instrumental, and practical method development in 2D-LC.
Case studies of 2D-LC use for protein characterization.

Main Results:

Significant theoretical and instrumental advancements support 2D-LC method development.
Powerful applications of 2D-LC have emerged for biotherapeutic protein analysis.
2D-LC effectively tackles challenges such as protein aggregation in complex formulations.

Conclusions:

2D-LC is a critical tool for modern bioanalysis, especially for complex biologics.
Continued development enhances the utility of 2D-LC for pharmaceutical characterization.
The technique provides essential solutions for analyzing challenging protein-based therapeutics.