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Dopamine biosensor based on surface functionalized nanostructured nickel oxide platform.

Appan Roychoudhury1, Suddhasatwa Basu2, Sandeep Kumar Jha1

  • 1Centre for Biomedical Engineering, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.

Biosensors & Bioelectronics
|December 3, 2015
PubMed
Summary
This summary is machine-generated.

A novel dopamine biosensor was created using nickel oxide nanoparticles and tyrosinase enzyme. This electrochemical sensor demonstrates high sensitivity and selectivity for detecting dopamine in real samples.

Keywords:
Dopamine biosensorElectrochemical sensorNeurochemical detectionNiO nanoparticlesTyrosinase

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Area of Science:

  • Electrochemistry
  • Nanomaterials Science
  • Biomedical Engineering

Background:

  • Dopamine is a crucial neurotransmitter implicated in various neurological functions and disorders.
  • Accurate and sensitive detection of dopamine is vital for clinical diagnostics and research.
  • Existing detection methods often face limitations in sensitivity, selectivity, or portability.

Purpose of the Study:

  • To develop a novel electrochemical biosensor for sensitive and selective dopamine detection.
  • To utilize nickel oxide nanoparticles (NiO NPs) functionalized with tyrosinase enzyme for enhanced sensing performance.
  • To validate the biosensor's efficacy using real biological samples.

Main Methods:

  • Nickel oxide nanoparticles (NiO NPs) were synthesized using a sol-gel method with sodium dodecyl sulphate (SDS) as a template.
  • Tyrosinase enzyme was conjugated onto the NiO NPs, and the conjugate was deposited onto an indium tin oxide (ITO) coated polyethylene terephthalate (PET) substrate.
  • Electrochemical detection was performed using the fabricated Tyrosinase/NiO/ITO electrode.
  • Structural and morphological characterization was done using XRD, TEM, DLS, AFM, and FTIR.

Main Results:

  • The NiO NPs were successfully synthesized with controlled size and morphology.
  • The Tyrosinase/NiO/ITO electrode exhibited high sensitivity (60.2 nA/µM) within a linear detection range of 2-100 µM.
  • The biosensor demonstrated a low detection limit (1.038 µM), rapid response time (45s), and good shelf life (45 days).
  • The sensor showed excellent selectivity in the presence of interfering substances and was successfully validated with real samples.

Conclusions:

  • The developed tyrosinase-functionalized NiO NPs platform offers a promising approach for sensitive and selective electrochemical detection of dopamine.
  • This biosensor exhibits significant potential for application in clinical diagnostics and monitoring of neurochemical levels.
  • The NiO NP-based biosensor platform can be extended for the detection of other catecholamines and phenolic compounds.