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Updated: Mar 29, 2026

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology
Brigitte Gasser1,2, Matthias G Steiger3,4, Diethard Mattanovich5,6
1Department of Biotechnology, BOKU-University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria. brigitte.gasser@boku.ac.at.
Abstract:
Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pichia pastoris (Komagataella sp.). Such a promoter collection can serve as an excellent toolbox for cell and metabolic engineering, and for gene expression to produce heterologous proteins.
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