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Preparation of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate analogs
Analytical Biochemistry
|February 15, 1989
Abstract:
A simple procedure for the bulk preparation of 20 mg of Escherichia coli elongation factor (EF)-Tu-GTP analogs is described. The protocol is based upon the preparation and stabilization of nucleotide-free EF-Tu using an EF-Ts affinity chromatographic resin. The procedure is a general one for the preparation of any GTP analog of EF-Tu.