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Updated: Mar 29, 2026
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
Published on: October 28, 2021
Interferon-λ1 suppresses invasion and enhances autophagy in human osteosarcoma cell
Dongmei Gao1, Jun Zhao2, Xiuyi Li1
1Department of Clinical Laboratory, Third Affiliated Hospital of Anhui Medical University Hefei 230032, China.
Objective:
The purpose of the present study was to determine whether type III IFN can modulate the autophagic response in human osteosarcoma cell.
Methods:
Human osteosarcoma cell were treated with Interferon-λ1. We investigated that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells by Matrigel invasion assay. Autophagy were assessed by acridine orange staining, MDC staining and Transmission electron microscopy.
Results:
In this study, we found that Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Acridine orange staining and MDC staining showed that Interferon-λ1 triggered the accumulation of acidic vesicular and autolysosomes in osteosarcoma cell. The acridine orange osteosarcoma cell ratios were 3.6 ± 0.5%, 4.5 ± 0.8%, and 12.4 ± 1.7% after treatment with 1, 10, and 100 ng/mL Interferon-λ1 for 48 h. Osteosarcoma cell cells treated with 100 ng/mL Interferon-λ1 for 48 h developed autophagy some-like characteristics, including single or double-membrane vacuoles containing intact and degraded cellular debris.
Conclusions:
Interferon-λ1 could inhibit the invasive ability of osteosarcoma cells. Autophagy can be induced in a dose-dependent manner by treatment with Interferon-λ1 in osteosarcoma cell.
Insights
Interferon-lambda1 (IFN-λ1) inhibits osteosarcoma cell invasion and induces autophagy in a dose-dependent manner. This study reveals IFN-λ1
Area of Science:
- Oncology
- Immunology
- Cell Biology
Background:
- Osteosarcoma is a primary bone malignancy with high metastatic potential.
- Autophagy plays a complex role in cancer progression and treatment response.
- Type III interferons (IFN-λs) are cytokines with antiviral and immunomodulatory functions.
Purpose of the Study:
- To investigate the effect of type III interferon (IFN-λ1) on the autophagic response in human osteosarcoma cells.
- To determine if IFN-λ1 can modulate the invasive capabilities of osteosarcoma cells.
Main Methods:
- Human osteosarcoma cells were treated with varying concentrations of Interferon-λ1 (IFN-λ1).
- Invasive ability was assessed using Matrigel invasion assays.
- Autophagy was evaluated through acridine orange staining, MDC staining, and Transmission Electron Microscopy (TEM).
Main Results:
- Interferon-λ1 significantly inhibited the invasive ability of osteosarcoma cells.
- IFN-λ1 treatment led to the accumulation of acidic vesicular organelles and autolysosomes, indicative of autophagy.
- Autophagy induction by IFN-λ1 was observed in a dose-dependent manner, with characteristic autophagosome-like structures seen at higher concentrations.
Conclusions:
- Interferon-λ1 demonstrates anti-invasive properties against human osteosarcoma cells.
- IFN-λ1 effectively induces autophagy in osteosarcoma cells, suggesting a potential therapeutic mechanism.
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