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MiR181c inhibits ovarian cancer metastasis and progression by targeting PRKCD expression

Lijuan Yao1, Li Wang1, Fengxia Li2

  • 1Department of Gynaecology and Obstetrics, Affiliated Hospital of Binzhou Medical College Binzhou 256603, China.

International Journal of Clinical and Experimental Medicine
|December 3, 2015
PubMed

Insights

MicroRNA-181c (miR-181c) is decreased in ovarian cancer, inhibiting tumor progression and metastasis by targeting protein kinase C delta (PRKCD). Lower miR-181c levels correlate with advanced stages and lymph node involvement.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • MicroRNAs (miRNAs) are key posttranscriptional regulators of gene expression.
  • Dysregulated miRNA expression is implicated in various cancers, including ovarian cancer.
  • Ovarian cancer progression and metastasis are complex processes influenced by genetic alterations.

Purpose of the Study:

  • To investigate the role of miR-181c in ovarian cancer.
  • To identify the downstream targets of miR-181c in ovarian cancer.
  • To elucidate the mechanism by which miR-181c affects ovarian cancer progression and metastasis.

Main Methods:

  • Quantitative real-time PCR to measure miR-181c expression in ovarian cancer tissues and cell lines.
  • In vitro functional assays (proliferation, migration, invasion) using A2780 cells.
  • Bioinformatics analysis and dual-luciferase reporter assays to identify and validate miR-181c targets.
  • Western blotting to assess protein expression levels.

Main Results:

  • miR-181c expression was significantly decreased in ovarian cancer tissues, particularly in those with lymph node metastasis and advanced pathological stage (IV).
  • Overexpression of miR-181c in A2780 cells inhibited proliferation, metastasis, and induced G1 phase arrest.
  • Protein kinase C delta (PRKCD) was identified as a direct target of miR-181c, with its expression inversely correlated with miR-181c levels in ovarian cancer samples.
  • Knockdown of PRKCD mimicked the effects of miR-181c, reducing cell proliferation, metastasis, and inducing G1 arrest.

Conclusions:

  • miR-181c acts as a tumor suppressor in ovarian cancer.
  • The miR-181c/PRKCD axis plays a critical role in regulating ovarian cancer cell proliferation, metastasis, and cell cycle progression.
  • miR-181c may serve as a potential therapeutic target or biomarker for ovarian cancer.
Keywords:
Ovarian cancerinvasion and metastasismiR-181c

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