Forkhead box protein M1 predicts outcome in human osteosarcoma

Area of Science:

• Oncology
• Molecular Biology
• Biomarker Discovery

Background:

• Osteosarcoma is a primary bone malignancy with significant impact on patient survival.
• Identifying reliable prognostic biomarkers is crucial for improving patient outcomes in osteosarcoma.

Purpose of the Study:

• To investigate the expression levels of FOXM1 (Forkhead box M1) in osteosarcoma.
• To determine the correlation between FOXM1 expression and clinicopathological factors and patient prognosis in osteosarcoma.

Main Methods:

• FOXM1 mRNA and protein expression were quantified using Real-time quantitative PCR (qRT-PCR) and Western blot.
• Immunohistochemistry (IHC) was employed to assess FOXM1 protein levels in 83 osteosarcoma tissue samples.
• Statistical analyses, including Kaplan-Meier and log-rank tests, were used to evaluate survival data.

Main Results:

• FOXM1 mRNA and protein levels were significantly elevated in osteosarcoma tissues compared to noncancerous bone tissues.
• High FOXM1 expression was significantly associated with poorer overall survival in osteosarcoma patients (P = 0.0001).

Conclusions:

• FOXM1 is upregulated in osteosarcoma.
• Elevated FOXM1 expression serves as an indicator of poor prognosis in osteosarcoma patients.
• FOXM1 demonstrates potential as a prognostic biomarker for osteosarcoma.