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Establishment of lymphatic filarial specific IgG4 indirect ELISA detection method
Jin Li1, Qing-Kuan Wei1, Shao-Liang Hu2
1Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Disease, WHO Collaborating Centre for Lymphatic Filariasis Jining 272033, China.
Objective:
To establish the lymphatic filarial specific IgG4 indirect ELISA detection method and develop the kits.
Methods:
ELISA and the developed specific IgG4 reagent was used to explore the best way for detecting filarial specific IgG4. Combined with the production process of commercialized enzyme immunoassay kit to develop economical lymphatic filarial specific IgG4 test kit, and to explore the value of the kit in the laboratory.
Results:
We determined the most optimal detective antigen was Malay adult filarial antigen and the optimal concentration of coating antigen was 1.0 μg/ml. The appropriate serum dilution was 1:20 to 40 and the work titers of specific IgG4 agents was 1:800. We determined the optimal reaction time for substrates and developed a reproducible and stable detection kit with sensitive and specificity, which was easy to operate.
Conclusion:
We successfully established the lymphatic filarial specific IgG4 indirect ELISA detection method and developed the kits with good reproducibility and stable result, which should be widely applied.
