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Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
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KCC2 Gates Activity-Driven AMPA Receptor Traffic through Cofilin Phosphorylation.

Quentin Chevy1, Martin Heubl1, Marie Goutierre1

  • 1Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche-S 839, F-75005, Paris, France, Sorbonne Universités, Université Pierre et Marie Curie Université Paris 06, Unité Mixte de Recherche-S 839, F-75005, Paris, France, Institut du Fer a Moulin, F-75005, Paris, France, and.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|December 4, 2015
PubMed
Summary

The neuronal K/Cl transporter KCC2 impacts glutamatergic synapses by regulating AMPA receptor delivery. Suppressing KCC2 disrupts synaptic plasticity via actin cytoskeleton changes in dendritic spines.

Keywords:
AMPA receptorKCC2STED microscopyactinspinesynaptic plasticity

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Synaptic Plasticity

Background:

  • Neuronal K/Cl transporter KCC2 expression is regulated by neuronal activity.
  • Altered KCC2 expression affects chloride homeostasis and GABA signaling.
  • Emerging evidence suggests KCC2 influences glutamatergic synapse development and function.

Purpose of the Study:

  • Investigate the role of KCC2 in glutamatergic synapse function.
  • Elucidate the mechanisms by which KCC2 affects synaptic plasticity.
  • Determine KCC2's impact on AMPA receptor trafficking and spine morphology.

Main Methods:

  • Utilized rat hippocampal neurons.
  • Suppressed KCC2 expression to observe effects on synaptic plasticity.
  • Assessed activity-driven AMPA receptor membrane delivery.
  • Analyzed Rac1/PAK- and LIMK-dependent cofilin phosphorylation and actin polymerization.

Main Results:

  • KCC2 suppression prevented long-term potentiation of glutamatergic synapses.
  • This effect was independent of KCC2 transporter activity.
  • KCC2 suppression led to increased cofilin phosphorylation and actin polymerization in dendritic spines.
  • Activity-driven AMPA receptor membrane delivery was inhibited.

Conclusions:

  • KCC2 plays a critical role in regulating the dendritic spine actin cytoskeleton.
  • KCC2 gates long-term plasticity at excitatory synapses in cortical neurons.
  • KCC2 influences synaptic plasticity through actin cytoskeleton dynamics, independent of its transporter function.