Microfluidic Generation of Porous Microcarriers for Three-Dimensional Cell Culture

Inspired by the microstructure of the stem cell niche, which is generally composed of adjacent cell protection layers and an extracellular matrix (ECM), we present novel microfluidic porous microcarriers for cell culture that consist of external-internal connected scaffold structures and biopolymer matrix fillers. The biomimetic scaffold structure of the porous microcarriers not only avoids the imposition of shear forces on the encapsulated cells but also provides a confined microenvironment for cell self-assembly, whereas the biopolymers in the porous cores of the microcarriers can act as an ECM microenvironment to promote the formation of multicellular spheroid aggregates for biomedical applications.