Related Experiment Video
Updated: Mar 29, 2026
11:32
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
7.5K
SerpinB3 and Yap Interplay Increases Myc Oncogenic Activity.
Cristian Turato1, Stefania Cannito2, Davide Simonato1
1Dept. of Medicine, University of Padova, Italy.
Scientific Reports
|December 5, 2015
Summary
SerpinB3 enhances liver cancer by increasing Myc production. It directly inhibits Myc cleavage and indirectly activates the Yap pathway, promoting tumor growth.
Area of Science:
- Hepatology
- Molecular Oncology
- Carcinogenesis research
Background:
- SerpinB3 is an early marker of liver carcinogenesis.
- Myc overexpression correlates with aggressive tumors.
- Yes-associated protein (Yap) regulates proliferation and is upregulated in hepatocellular carcinomas.
Purpose of the Study:
- To investigate the interplay between SerpinB3 and Myc in liver cancer.
- To characterize the functional modulation of Myc by SerpinB3.
Main Methods:
- Utilized transgenic mice and hepatoma cells overexpressing SerpinB3.
- Investigated molecular mechanisms involving calpain and the Hippo pathway.
- Performed in vitro inhibition assays with human recombinant SerpinB3.
Main Results:
- SerpinB3 up-regulated Myc through calpain and Hippo-dependent pathways.
- SerpinB3 inhibited Calpain activity in vitro, reducing Myc cleavage.
- SerpinB3 indirectly increased Myc transcription via Yap pathway activation.
Conclusions:
- SerpinB3 promotes Myc production through direct and indirect mechanisms.
- Inhibition of Myc cleavage and Yap pathway activation are key roles of SerpinB3 in liver cancer.
Related Concept Videos
02:23Abnormal Proliferation
5.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
01:06Induced Pluripotent Stem Cells
6.2K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
6.2K
01:19Interactions Between Signaling Pathways
7.9K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
7.9K
03:03mTOR Signaling and Cancer Progression
5.1K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
5.1K
03:03mTOR Signaling and Cancer Progression
1.7K
1.7K
01:22PI3K/mTOR/AKT Signaling Pathway
6.3K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
6.3K