Related Experiment Video
Updated: Mar 29, 2026

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
Published on: June 29, 2017
Metabolic Engineering of Pseudomonas putida KT2440 to Produce Anthranilate from Glucose
Jannis Kuepper1, Jasmin Dickler1, Michael Biggel1
1Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt), Rheinisch-Westfälische Technische Hochschule Aachen University Aachen, Germany.
Abstract:
The Pseudomonas putida KT2440 strain was engineered in order to produce anthranilate (oAB, ortho-aminobenzoate), a precursor of the aromatic amino acid tryptophan, from glucose as sole carbon source. To enable the production of the metabolic intermediate oAB, the trpDC operon encoding an anthranilate phosphoribosyltransferase (TrpD) and an indole-3-glycerol phosphate synthase (TrpC), were deleted. In addition, the chorismate mutase (pheA) responsible for the conversion of chorismate over prephenate to phenylpyruvate was deleted in the background of the deletion of trpDC to circumvent a potential drain of precursor. To further increase the oAB production, a feedback insensitive version of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase encoded by the aroG (D146N) gene and an anthranilate synthase (trpE (S40F) G) were overexpressed separately and simultaneously in the deletion mutants. With optimized production conditions in a tryptophan-limited fed-batch process a maximum of 1.54 ± 0.3 g L(-1) (11.23 mM) oAB was obtained with the best performing engineered P. putida KT2440 strain (P. putida ΔtrpDC pSEVA234_aroG (D146N) _trpE (S40F) G).
More Related Videos
Related Concept Videos
Production of Antibiotics
Bioreactor Controls-III
Production of Pharmaceuticals
Amino Acid Biosynthetic Pathways
Other Glycolytic Pathways
Biosynthesis in Bacteria

