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Updated: Mar 29, 2026

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
Published on: June 24, 2016
Production of biodiesel from coastal macroalgae (Chara vulgaris) and optimization of process parameters using
Shaila Siddiqua1, Abdullah Al Mamun1, Sheikh Md Enayetul Babar1
1Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh.
Abstract:
Renewable biodiesels are needed as an alternative to petroleum-derived transport fuels, which contribute to global warming and are of limited availability. Algae biomass, are a potential source of renewable energy, and they can be converted into energy such as biofuels. This study introduces an integrated method for the production of biodiesel from Chara vulgaris algae collected from the coastal region of Bangladesh. The Box-Behnken design based on response surface methods (RSM) used as the statistical tool to optimize three variables for predicting the best performing conditions (calorific value and yield) of algae biodiesel. The three parameters for production condition were chloroform (X1), sodium chloride concentration (X2) and temperature (X3). Optimal conditions were estimated by the aid of statistical regression analysis and surface plot chart. The optimal condition of biodiesel production parameter for 12 g of dry algae biomass was observed to be 198 ml chloroform with 0.75 % sodium chloride at 65 °C temperature, where the calorific value of biodiesel is 9255.106 kcal/kg and yield 3.6 ml.
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