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Characterization and cloning of MwoI (GCN7GC), a new type-II restriction-modification system from Methanobacterium
K D Lunnen1, R D Morgan, C J Timan
1New England Biolabs, Inc., Beverly, MA 01915.
Gene
|April 15, 1989
Abstract:
R.MwoI, a type-II restriction enzyme with the new specificity 5'-GCN7GC-3', was found in extracts of the thermophilic archaebacterium, Methanobacterium wolfei. R.MwoI cleaves duplex DNA producing fragments with 3-nt, 3'-terminal extensions, thus: GCN5/N2GC. The genes coding for the MwoI restriction and modification enzymes were cloned into Escherichia coli on the plasmid vector pBR322. The clones synthesize a low level of R.MwoI endonuclease. The plasmids display incomplete MwoI-specific modification, suggesting that the clones synthesize a low level of the M.MwoI methyltransferase, too.