Aquitalea pelogenes sp. nov., isolated from mineral peloid

Strain P1297^T was isolated in the frame of a project aimed on the psychrotolerant microbiota occurring in water sources. The strain initially identified as a tentative species of the genus Aeromonas was rod-shaped, Gram-stain-negative, facultatively anaerobic and oxidase-positive. Subsequently, 16S rRNA gene sequence analysis placed strain P1297^T within the class Betaproteobacteria and showed Aquitalea magnusonii TRO-001DR8^T as the closest phylogenetic relative with 99.28 % 16S rRNA gene sequence similarity. Digital DDH and average nucleotide identity (ANI) were determined to evaluate the genomic relationship between strain P1297^T and Aquitalea magnusonii CCM 7607^T. Digital DDH estimation (31.3 ± 2.46 %) as well as ANI (85.6001 %; reciprocal value 85.3277 %) proved the dissimilarity of strain P1297^T. Further investigation using phenotyping, automated ribotyping, whole-cell protein profiling and PCR-fingerprinting methods showed a distinct taxonomic position of strain P1297^T among hitherto described species of the genus Aquitalea. DNA-DNA hybridization experiments revealed low binding values between strain P1297^T and Aquitalea magnusonii CCM 7607^T (57 ± 3 %) and Aquitalea denitrificans CCM 7935^T (41 ± 5 %). The DNA G+C content of strain P1297^T was 60.3 mol%. The predominant fatty acids were C_16 : 1ω7c/ iso-C_15 : 0 2-OH (47.0 %), C_16 : 0 (24.5 %) and C_18 : 1ω7c (10.6 %), and the quinone system contained predominantly ubiquinone Q-8. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids and one unidentified aminophospholipid. Obtained results of genotypic and chemotaxonomic methods clearly proved that strain P1297^T represents a novel species of the genus Aquitalea, for which the name Aquitalea pelogenes sp. nov. is proposed. The type strain is P1297^T ( = CCM 7557^T = LMG 28989^T = CCUG 67440^T).