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The post-antibiotic effect defined by bacterial morphology.

V Lorian1, J Ernst, L Amaral

  • 1Department of Epidemiology and Infection Control, Bronx Lebanon Hospital Center, New York.

The Journal of Antimicrobial Chemotherapy
|April 1, 1989
PubMed
Summary
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The post-antibiotic effect (PAE) in Escherichia coli was studied using ciprofloxacin and ampicillin. Morphological changes and growth kinetics revealed varying PAE durations for each antibiotic, impacting bacterial enzyme activity.

Area of Science:

  • Microbiology
  • Pharmacology
  • Bacteriology

Background:

  • Antibiotics like ciprofloxacin and ampicillin inhibit bacterial enzyme activity.
  • Upon antibiotic removal, bacterial enzyme synthesis resumes, defining the post-antibiotic effect (PAE).
  • Bacterial morphology changes reflect enzymatic activity and can be used to assess the PAE.

Purpose of the Study:

  • To determine the post-antibiotic effect (PAE) of ciprofloxacin and ampicillin on Escherichia coli.
  • To compare PAE measurements using bacterial morphology and growth kinetics.
  • To investigate the relationship between antibiotic exposure, enzyme inactivation, and bacterial recovery.

Main Methods:

  • Incubation of Escherichia coli with ciprofloxacin or ampicillin.
  • Observation of bacterial filamentation as a morphological indicator.

Related Experiment Videos

  • Measurement of PAE using morphological criteria (10% filaments) and growth kinetics.

    • Main Results:

    • Both antibiotics induced significant bacterial filamentation (>90% after 5h).
    • Morphological PAE was 4 hours for ciprofloxacin and 3 hours for ampicillin.
    • Growth kinetics revealed a PAE of 2 hours for ciprofloxacin and 0 hours for ampicillin.

    Conclusions:

    • Bacterial morphology and growth kinetics provide different measures of the post-antibiotic effect.
    • Ciprofloxacin exhibited a longer post-antibiotic effect compared to ampicillin across both methods.
    • The study highlights the complex relationship between antibiotic exposure and bacterial recovery dynamics.