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Effect of temperature on platelet adherence.

S Braune1,2, G M Fröhlich, A Lendlein1,2,3

  • 1Institute of Biomaterial Science, Helmholtz-Zentrum Geesthacht, Teltow, Germany.

Clinical Hemorheology and Microcirculation
|December 8, 2015
PubMed
Summary

Investigating temperature effects on biomaterial thrombogenicity, this study found that platelet adhesion varied between polymers like PDMS and PET at 22°C and 37°C, but PTFE showed similar results. Overall polymer thrombogenicity was comparable across temperatures.

Keywords:
Biomaterialplatelet activationplatelet adhesiontemperaturethrombogenicity

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Area of Science:

  • Biomaterials Science
  • Hemocompatibility Testing
  • Surface Science

Background:

  • Thrombogenicity is a key in vitro parameter for assessing artificial surface hemocompatibility.
  • While temperature effects on platelet aggregation are known, its impact on platelet adhesion to biomaterials is less explored.
  • Understanding temperature influence on platelet adhesion is crucial for biomedical device handling.

Purpose of the Study:

  • To analyze the influence of two incubation temperatures (22°C and 37°C) on platelet adhesion to different biomaterials.
  • To compare the thrombogenicity of poly(dimethyl siloxane) (PDMS), polytetrafluoroethylene (PTFE), and polyethylene terephthalate (PET) at varying temperatures.

Main Methods:

  • In vitro static test evaluating thrombogenicity of PDMS, PTFE, and PET.
  • Platelet adhesion analysis using blood from healthy subjects.
  • Incubation of platelet-rich plasma (PRP) with polymers at 22°C and 37°C.
  • Assessment of surface-adherent platelets via fluorescent labeling and image-based analysis.

Main Results:

  • Significant differences in platelet adhesion density were observed between 22°C and 37°C for PDMS and PET.
  • PTFE, a highly thrombogenic polymer, exhibited similar platelet adhesion levels at both temperatures.
  • Analysis of covered surface area per platelet indicated no difference in platelet activation state between the two temperatures for any polymer.

Conclusions:

  • Platelet adhesion to PDMS and PET is temperature-dependent, unlike PTFE.
  • Despite temperature-related variations in platelet adhesion for some polymers, the overall thrombogenicity of the investigated materials was comparable at 22°C and 37°C.
  • The study highlights the nuanced effect of temperature on biomaterial-platelet interactions.